skincaremanual.org

SAIC-Frederick, Fluidigm collaborate to decode entire genome of Epstein-Barr virus

March 09, 2017

"Our team was able to target, capture and amplify the entire EBV genome from human DNA samples in an easy and cost-effective manner prior to sequencing," said Amy Hutchinson, Director of Operations for NCI's Core Genotyping Facility. Hutchinson is employed by SAIC-Frederick, the contractor that operates the facility for NCI.

Conventional DNA amplification techniques require three steps before gene sequencing can begin - one step to combine samples and reagents for amplification, a second step to make enough copies (amplification) for sequencing, and a third step to attach sequence adaptors prior to sequencing. The Fluidigm Access Array technology combines these steps into one. This results in a significant savings in terms of starting material required, research time and overall project costs.

"Fluidigm's Access Array technology is the easiest, most cost-effective and consistent way to amplify and prepare multiple samples for targeted resequencing. When combined with almost any next-generation sequencer, researchers can efficiently explore diseases and decipher the genetic codes that might hold the keys to prevention or cure," said Gajus Worthington, Fluidigm President and Chief Executive Officer.

SOURCE SAIC-Frederick, Inc. and Fluidigm Corporation